Once antigen is provided, library panning is performed to select for binders to a target of interest.
ELISAs can be used to demonstrate that we have found isolated binders that perform comparably to reference antibody standards.
Single-domain antibody service
Provide us your purified target of interest.
We will screen our synthetic VHH library of single-domain antibodies (sdAbs) for binders. This service includes:
3 rounds of panning against the protein immobilized on a plate
ELISA plate screening of 94 nanobody clones from the third round of panning
Sequencing of up to the top 6 binders
benefits of our approach
Animal Free, High Diversity: Our library is fully synthetic with more diversity than a naive animal library
Speed: We can go from antigen to VHH sequences in as little as 3-4 weeks
Cost: Single target screening starting at $10k
VHH Advantages: Single-domain antibodies are small (15 kDa) while retaining high stability and high affinity
Customization: Custom tags and other modifications (e.g. fluorophores) available
why QBI?
Established in 2010, QBI has been providing synthetic biology and sensor services for over 14 years. We have successfully delivered on over 20 projects with funding from NIH, DOE, DARPA, IARPA, USDA, and EPA.
Download our poster for more background information.